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建立慢性应激抑郁大鼠模型,采用ELISA法测定5-HT、NA、DA水平,ICH法测定BDNF、Trk B、p CREB、p ERK的表达,评价三七叶总皂苷对脑内单胺及神经因子的影响。建模五周后,与空白组比较,模型组大鼠体重、糖水偏好率分别降低了17.43%、39.49%,自主活动表现为探索能力降低、趋避能力升高,海马体质量百分比显著减少,呈现出人类抑郁症中体重下降、快感缺失、自主活动下降、海马体萎缩等核心症状。而给药组与模型组比较,上述指标得到有效逆转。与模型组比较,三七叶总皂苷高剂量组(100mg/kg)可以使脑内NA、DA水平分别升高13.89%及19.66%,而对5-HT水平没影响,并且使脑内BDNF、Trk B、p CREB、p ERK的表达分别上升16.67%、47.83%、20.42%、22.84%,表明三七叶总皂苷具有良好的抗抑郁作用。
The levels of 5-HT, NA and DA were determined by ELISA. The expressions of BDNF, Trk B, p CREB and p ERK were determined by ICH, Effect of nerve factors. After five weeks of modeling, compared with the blank group, the body weight and sugary preference rate of the model group decreased by 17.43% and 39.49%, respectively. The autonomous activity manifested as decreased exploration ability, increased avoidance ability, significantly decreased percentage of hippocampal body mass Depression in human body weight loss, loss of pleasure, decreased autonomy, atrophy of the hippocampus and other core symptoms. The administration group and model group, the above indicators have been effectively reversed. Compared with model group, the high dose of total saponins of Panax Notoginseng (100mg / kg) increased NA and DA levels in brain by 13.89% and 19.66% respectively, but had no effect on 5-HT level, and increased BDNF, The expression of Trk B, p CREB and p ERK increased by 16.67%, 47.83%, 20.42% and 22.84%, respectively, which indicated that the total saponin of Panax notoginseng had good antidepressant effect.