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目的通过对紫苏种子萌发及幼苗生理特性的研究,寻找提高紫苏种子及幼苗在盐胁迫下抗性能力的途径。方法测定盐胁迫下紫苏种子在不同浓度的外源5-氨基乙酰丙酸(ALA)处理后,发芽势、发芽率、发芽指数和活力指数的变化,并对紫苏幼苗叶片的相对含水量、幼苗总生物量、可溶性糖量、叶片丙二醛(MDA)量以及超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性进行测定。结果 100 mmol/L NaCl胁迫下的紫苏种子萌发受到显著抑制,但是经过不同质量浓度的ALA处理后,各项萌发指标均有所升高,其中经过50 mg/L ALA处理后效果最为显著,各项指标均达到最大值,发芽势为71.3%,发芽率为90.5%,发芽指数为15.9,活力指数为0.129 6。各处理均提高了幼苗总生物量和可溶性糖的量,减缓了盐胁迫下紫苏叶片相对含水量下降的趋势,降低了叶片MDA的量,显著地提高了SOD、POD和CAT 3种酶的活性,且经过50 mg/L ALA处理后3种酶的活性分别达到最大值(0.79、7.69、5.84 U/mg)。结论 50 mg/L ALA能够有效地减缓盐胁迫对紫苏种子及幼苗产生的伤害,提高种子及幼苗的抗盐能力。
Aim To study the ways to improve the resistance of perilla seeds and seedlings under salt stress by studying the germination of seed and the physiological characteristics of seedlings. Methods The changes of germination energy, germination rate, germination index and vigor index of Perilla frutescens seeds under different concentrations of exogenous 5-aminolevulinic acid (ALA) under salt stress were measured. The relative water content , Total seedling biomass, soluble sugar content, malondialdehyde (MDA) content, and activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) Results The germination of Perilla frutescens seeds under 100 mmol / L NaCl stress was significantly inhibited. However, all the germination indexes increased after treatment with different concentrations of ALA. After 50 mg / L ALA treatment, the effect was the most significant, The indexes all reached the maximum, the germination potential was 71.3%, the germination rate was 90.5%, the germination index was 15.9 and the vigor index was 0.129 6. All the treatments increased the total biomass and soluble sugar content of seedlings, reduced the relative water content of Perilla frutescens leaves under salt stress, decreased the content of MDA and significantly increased the activities of SOD, POD and CAT The activities of the three enzymes reached the maximum (0.79, 7.69, 5.84 U / mg) after 50 mg / L ALA treatment. Conclusions 50 mg / L ALA can effectively reduce salt damage to Perilla frutescens seeds and seedlings, and improve salt tolerance of seeds and seedlings.