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以转基因的抗晚疫病型马铃薯(Solanum tuberosum)株系DR1、DR3a和野生型株系DG为材料,通过半定量RT-PCR研究了接种晚疫病病原菌(Phytophthora infestans)生理小种89148-9后叶片中水杨酸和乙烯合成途径关键酶苯丙氨酸解胺酶(PAL)基因(poPAL)和1-氨基环丙烷-1-羧酸(1-Aminocyclopropane-1-carboxylic acid,ACC)合成酶(ACS)基因(poACS)的表达情况。结果表明,接种后3个株系都能诱导poPAL和poACS基因的表达,但大部分转基因株系叶片内该基因的诱导表达量高于野生型,并且其表达峰值多数早于野生型。说明poPAL和poACS基因可能参与了马铃薯的抗晚疫病反应,但不同株系中这些基因的诱导表达模式不同,这可能是不同马铃薯株系抗、感晚疫病的原因所在。
The transgenic plants of Solanum tuberosum strain DR1, DR3a and wild-type DG were used to study the effects of inoculating the leaves of the late-onset pathogen 89148-9 The key enzymes in the synthesis of salicylic acid and ethylene are the enzyme (PAL) gene (poPAL) and 1-aminocyclopropane-1-carboxylic acid (ACC) synthase ACS) gene (poACS) expression. The results showed that all the three inoculated plants could induce the expression of poPAL and poACS genes. However, most of the transgenic lines induced higher expression of poPAL and poACS genes than the wild type, and most of the expression peaks were earlier than the wild type. The results showed that poPAL and poACS genes may be involved in the resistance to late blight of potato, but different expression patterns of these genes in different strains may be the reason of resistance and late blight of different potato lines.