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分别采用邻苯二甲醛柱前衍生高效液相色谱(HPLC)荧光检测法(简称OPA法)和丹磺酰氯柱前衍生HPLC紫外检测法(简称DNS-Cl法)测定水产品中生物胺含量,比较这两种方法对水产品中多种生物胺检测的差异。结果表明,两种方法对生物胺的检测质量浓度范围在1~100 mg/L,OPA法的检出限(0.03~0.35 mg/kg)略优于DNS-Cl法(0.15~0.90 mg/kg)。方法的精确度试验表明:OPA法的加标回收率(76.2%~107%)略优于DNSCl法(62.2-118%),然而两种方法的测量精密度均小于10%(OPA法1.3%~6.7%,DNS-Cl法2.6%~9.9%,n=6)。OPA法操作简便、快速,然而其衍生产物不太稳定,对亚精胺和精胺的检测灵敏度低;而DNS-Cl法操作相对繁琐,其衍生产物稳定,可同时测定包括亚精胺和精胺在内的多种生物胺。用这两种衍生方法检测水产品中组胺、腐胺、酪胺和色胺存在显著性差异(P<0.05)。
The biogenic amines contents of aquatic products were determined by HPLC-fluorescence spectrometry (OPA method) and Dansyl chloride pre-column derivatization HPLC (referred to as DNS-Cl method) The differences between these two methods in the detection of multiple biogenic amines in aquatic products were compared. The results showed that the detection limits of biogenic amines in the two methods ranged from 1 to 100 mg / L, and the detection limits of OPA method (0.03 ~ 0.35 mg / kg) were slightly better than those of DNS - Cl method (0.15 ~ 0.90 mg / kg ). The precision test showed that the spiked recoveries (76.2% -107%) of OPA method were slightly better than that of DNSCl method (62.2-118%), however, the precision of OPA method was less than 10% (OPA method 1.3% ~ 6.7%, DNS-Cl method 2.6% ~ 9.9%, n = 6). The OPA method is simple and rapid to operate. However, its derivatives are not very stable and have low sensitivity to spermidine and spermine. However, the DNS-Cl method is relatively complicated and its derivatives are stable. Simultaneous determination of spermidine and spermine Amines, including a variety of bio-amine. Histamine, putrescine, tyramine and tryptamine detected by these two methods were significantly different (P <0.05).