本文采用二阶导数分光光度法测定诺氟沙星葡萄糖注射液中诺氟沙星的含量。本法可排除葡萄糖及其分解产物5-羟甲基糠醛的干扰。在2—18μg/ml浓度范围内线性良好,r=0.99998,重现性好。日内日间变异系数分别为0.60%及0.72%。其方法回收率为100,0±0.50%(n=9)。测定了6批样品的含量,结果较为满意。 In this paper, the second derivative spectrophotometric determination of norfloxacin glucose injection of norfloxacin content. This law can rule out the interference of glucose and its decomposition products 5-hydroxymethyl furfural. Good linearity in the range of 2-18μg / ml, r = 0.99998, good reproducibility. The intra-day and inter-day variation coefficients were 0.60% and 0.72% respectively. The method recovery was 100,0 ± 0.50% (n = 9). The contents of 6 batches of samples were determined and the results were satisfactory.