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目的调查甘肃省河西走廊地区蚊虫种类及所携带虫媒病毒分布状况。方法使用诱蚊灯于18:00至次日06:00进行蚊虫标本采集,分类鉴定后液氮保存并运送至实验室备检;通过组织培养法分离病毒,并对病毒分离物进行血清学和分子生物学鉴定;利用生物信息学技术对新分离病毒的序列进行分析,完成同源性和系统发生分析。结果 2011年8月在甘肃省河西走廊地区7个县(市)共采集到蚊虫标本3属6种24028只,其中刺扰伊蚊最多,占采集总数的32.80%(7880/24028),淡色库蚊占30.26%(7272/24028);通过细胞培养分离到1株可以引起两种细胞(BHK-21和C6/36细胞)病变的病毒分离株(GS11-155);此外在32批蚊虫标本中检测到病毒(库蚊黄病毒、盖塔病毒、辽宁病毒)基因阳性,对其中11批核苷酸序列测定和分析发现,1批为蚊传黄病毒,3批为盖塔病毒,7批为辽宁病毒。结论甘肃省河西走廊地区优势蚊种为刺扰伊蚊,且蚊虫携带多种虫媒病毒,包括蚊传黄病毒、盖塔病毒和辽宁病毒。
Objective To investigate the species of mosquitoes and the distribution of arbovirus in Hexi Corridor, Gansu Province. Methods The mosquitoes were collected from 18:00 to 06:00 the next day by mosquito lamp. After classification and identification, the samples were stored in liquid nitrogen and sent to the laboratory for testing. The virus was isolated by tissue culture method and the isolates were subjected to serological and Molecular biological identification; the use of bioinformatics technology to analyze the sequence of the newly isolated virus to complete the homology and phylogenetic analysis. Results In August 2011, a total of 24028 mosquito species were collected from 7 species (or cities) of Hexi Corridor in Gansu Province, of which 6 were mosquitoes, of which 6 were mosquitoes, of which 62 were mosquitoes, accounting for 32.80% (7880/24028) of the total, The mosquito accounted for 30.26% (7272/24028) of mosquitoes. One isolate (GS11-155) was isolated by cell culture which could induce the pathological changes of both cells (BHK-21 and C6 / 36) Detection of the virus (Culex pipiens virus, Geida virus, Liaoning virus) gene positive, of which 11 batches of nucleotide sequence determination and analysis found that 1 batch of mosquito flavivirus, 3 batches of Geeta virus, 7 batches of Liaoning virus. Conclusion The predominant mosquito species in the Hexi Corridor in Gansu Province were Aedes albopictus mosquitoes, and the mosquitoes carried a variety of arbovirus, including the mosquito-borne flavivirus, Geeta virus and Liaoning virus.