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目的研究基质金属蛋白酶9(MMP9)基因多态性与中国云南汉族人群冠心病(CHD)的相关性。方法采用Sequenom Mass ARRAY~对云南省第二人民医院2012年9月至2015年9月收集的198例正常人(对照组)和514例冠心病患者(冠心病组)MMP9基因rs2274755单核苷多肽(SNP)位点的多态性进行基因分型,分析该基因多态性与冠心病的相关性。结果正常对照组和冠心病组rs2274755位点基因型频率分别为GG 0.67/0.76,GT 0.31/0.22,TT 0.02/0.02,差异有统计学意义(P<0.05),两组等位基因频率分别为G 0.82/0.87,T 0.18/0.13,差异有统计学意义(P<0.05)。校正年龄、性别、体质量指数等logistic回归分析显示,rs2274755位点携带T等位基因者患冠心病风险降低[加性模型(T vs G):OR=0.72,95%CI(0.52~0.99),P=0.046;显性模型(TT/GT vs GG):OR=0.96,95%CI(0.93~1.00),P=0.028]。结论 MMP9基因多态性与云南汉族人群冠心病发生风险相关。
Objective To investigate the relationship between MMP9 gene polymorphism and coronary heart disease (CHD) in Yunnan Han Chinese population. Methods A total of 198 normal subjects (control group) and 514 coronary heart disease patients (coronary heart disease group) collected from September 2012 to September 2015 in Yunnan Second People’s Hospital from September to September 2015 were analyzed by Sequenom Mass ARRAY ~ 单. Polymorphism of SNPs was genotyped and the association between the polymorphism and coronary heart disease was analyzed. Results The genotype frequency of rs2274755 in control group and CHD group were respectively GG 0.67 / 0.76, GT 0.31 / 0.22, TT 0.02 / 0.02, the difference was statistically significant (P <0.05). The allele frequencies of two groups were G 0.82 / 0.87, T 0.18 / 0.13, the difference was statistically significant (P <0.05). Logistic regression analysis showed that the rs2274755 locus had a lower risk of coronary heart disease than the T allele (OR vs 0.72, 95% CI, 0.52-0.99) , P = 0.046; TT / GT vs. GG: OR = 0.96, 95% CI 0.93-1.00, P = 0.028]. Conclusion MMP9 gene polymorphism is associated with the risk of coronary heart disease in Yunnan Han population.