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本文报告了使用不同抗原的包虫病间接血凝试验方法的实验结果。采用高浓度戊二醛化和1/20000鞣酸处理的绵羊红细胞,用绵羊肝包囊液粗抗原和部分纯化抗原致敏。用包虫病人血清进行方阵滴定,包囊液粗抗原,亲和层析抗原、Oriol氏法纯化抗原和Burstein氏法纯化抗原的最适致敏浓度分别为250、25、25和6.25μg/ml。血清终点稀释度的倒数皆为1024。用包囊液粗抗原对1295名健康人血清做间接血凝试验(IHA)结果,抗体滴度≥64者仅6例(0.46％)。95例牛肉绦虫病人血清的阳性率为1.05％(1/95)。这种致敏红细胞的冻干制剂,在室温下存放18个月后,仍保持稳定。囊液粗抗原、亲和层析抗原、Oriol氏法纯化抗原和Burstein氏法纯化抗原致敏红细胞,对47例包虫病人血清做IHA结果阳性率各为91.49％、44.68％、76.59％和85.12％,阳性血清抗体滴度的几何均值分别为389.29±2.55、247.69±4.20、271.21±2.45和343.65±2.43。29份囊虫病人血清IHA的结果为囊液粗抗原致敏红细胞的阳性率为27.5％,阳性血清的几何均值为128。其他三种抗原致敏的红细胞均为阳性。说明三种纯化抗原致敏红细胞与囊虫病人血清不发生交叉反应。根据本项实验结果建议将Burstein氏法纯化抗原致敏绵羊红细胞的间接血凝试验做为常规方法。
This paper reports the experimental results of the indirect hemagglutination test using different antigens of hydatid disease. Sheep red blood cells were treated with high glutaraldehyde and 1/20000 tannic acid and sensitized with sheep liver cyst fluid crude antigen and partially purified antigen. The optimal sensitization concentrations of e antigenic antigen and Burstein’s method for antigen purification were 50, 25, 25 and 6.25μg / ml. Serum end point dilution of all 1024. Indirect hemagglutination test (IHA) was performed on 1295 healthy human serum with crude antigen of cyst fluid. Only 6 patients (0.46%) had antibody titers ≥64. The positive rate of serum of 95 cases of beef taeniasis was 1.05% (1/95). The lyophilized preparations of this sensitized erythrocyte remain stable after 18 months of storage at room temperature. The positive rates of IHA in serum of 47 patients with hydatid disease were 91.49%, 44.68%, 76.59% and 85.12%, the geometric mean of positive serum antibody titers were 389.29 ± 2.55, 247.69 ± 4.20, 271.21 ± 2.45 and 343.65 ± 2.43.29, respectively. Serum IHA in cysticercosis patients was the positive rate of erythrocytes Was 27.5%, the geometric mean of positive serum was 128. The other three antigen-sensitized red blood cells were positive. It shows that three kinds of purified antigen-sensitized erythrocytes do not cross-react with cysticercosis patients. According to the results of this experiment, the indirect hemagglutination test of Burstein’s purified antigen-sensitized sheep erythrocytes is suggested as a routine method.