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目的：观察甲基莲心碱（Neferine，Nef）对阿霉素（ADM）抑制人急性粒细胞性白血病（HL60）细胞增殖作用的影响。方法：采用MTT比色法测定Nef及Nef与ADM合用对HL60细胞增殖的影响，同时应用软琼脂培养克隆形成法检Nef及Nef与ADM合用对HL60细胞克隆形成能力的作用。结果：1、MTT比色法测定：Nef（12．5μg·ml-）能增强ADM抑制HL60细胞增殖（P＜0．05）。2、软琼脂培养克隆形成法检测：Nef（12．5μg）亦能提高ADM的克隆抑制率（P＜0．05）。结论：Nef具有增强ADM抑制HL60细胞增殖和克隆形成能力作用。
OBJECTIVE: To observe the effect of Neferine (Nef) on adriamycin (ADM) inhibiting the proliferation of human acute myelogenous leukemia (HL60) cells. Methods: MTT colorimetric assay was used to determine the effect of Nef and Nef combined with ADM on the proliferation of HL60 cells, and soft agar culture colony formation assay was used to detect the effect of Nef and Nef on the colony forming ability of HL60 cells. The results: 1, MTT colorimetric assay: Nef (12.5μg · ml-) can enhance ADM inhibition of HL60 cell proliferation (P <0.05). 2, soft agar culture colony formation assay: Nef (12.5μg) can also increase the inhibition rate of ADM (P <0.05). Conclusion: Nef can enhance the proliferation and colony formation of HL60 cells induced by ADM.