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目的:探讨三元复合因子Net对人胰腺癌裸鼠移植瘤生长的抑制作用。方法:以聚合酶链反应(PCR)扩增Net基因全长片段,应用基因重组技术构建Ad5/F35-Net重组腺病毒载体。建立24只人胰腺癌裸鼠移植瘤模型,并随机分为PBS组、绿色荧光蛋白(GFP)组和Net组,3组分别注射0.1 mL的PBS、Ad5/F35-GFP(1×108PFU)和Ad5/F35-Net(1×108PFU),干预3周后,测定各组的移植瘤体积和质量,并应用免疫组化法检测各组移植瘤增殖细胞核抗原(PCNA)表达及移植瘤组织原位细胞凋亡情况。结果:本研究成功构建了表达Net的重组腺病毒载体。与PBS组及GFP组相比,Net组裸鼠移植瘤体积和质量明显减小(P均<0.05),而其PCNA表达显著降低(P<0.01),原位细胞凋亡明显增加(P<0.01)。结论:Net可明显抑制人胰腺癌裸鼠移植瘤的生长,抑制移植瘤细胞的增殖,促进移植瘤细胞原位凋亡。
Objective: To investigate the inhibitory effect of ternary complex factor Net on the growth of transplanted human pancreatic cancer in nude mice. Methods: The full-length fragment of Net gene was amplified by polymerase chain reaction (PCR), and Ad5 / F35-Net recombinant adenovirus vector was constructed by gene recombination technology. Twenty-four human pancreatic cancer xenografts were established and randomly divided into PBS group, GFP group and Net group. Three groups were injected with 0.1 mL of PBS, Ad5 / F35-GFP (1 × 108 PFU) and Ad5 / F35-Net (1 × 108 PFU). After 3 weeks of intervention, the volume and the mass of the transplanted tumor in each group were determined. The expression of PCNA and the location of the transplanted tumor in each group were detected by immunohistochemistry Apoptosis. Results: The recombinant adenovirus vector expressing Net was successfully constructed in this study. Compared with PBS group and GFP group, the volume and quality of transplanted tumor in nude mice were significantly decreased (P <0.05), and the expression of PCNA was significantly decreased (P <0.01), and the apoptosis in situ was significantly increased (P < 0.01). Conclusion: Net can significantly inhibit the growth of human pancreatic cancer xenografts in nude mice, inhibit the proliferation of xenograft tumor cells and promote the in situ apoptosis of xenograft tumor cells.