目的 :构建大鼠钠尿肽 B受体 c DNA探针克隆载体 ,为研究该受体基因表达奠定基础。方法 :设计钠尿肽 B受体特异的 PCR引物 ,提取大鼠肺组织总 RNA,进行反转录 PCR,扩增目的片段 ,回收目的片段 ,将其克隆入p GEM- T载体 ,酶切鉴定并用双脱氧法测序。结果 :PCR反应产物经琼脂糖凝胶电泳鉴定与所设计引物大小一致 ,克隆入 p GEM- T载体后酶切可切出所需片段 ,测序证实为所设计序列。结论 :构建的 p GEM- T重组质粒为进一步研究该受体基因表达、分布等创造了条件 OBJECTIVE: To construct cloning vector of rat natriuretic peptide B receptor c DNA probe and lay the foundation for the study of gene expression of this receptor. Methods: The specific primers of natriuretic peptide B receptor were designed and the total RNA was extracted from lung tissue of rats. Reverse transcription PCR was performed to amplify the target fragment. The target fragment was recovered and cloned into p GEM-T vector. And dideoxy method sequencing. Results: The PCR products were identified by agarose gel electrophoresis and the size of the designed primers was consistent. After cloning into p GEM-T vector, the desired fragment was cut and confirmed to be the designed sequence. CONCLUSION: The constructed p GEM-T recombinant plasmid has created the conditions for further study on the gene expression and distribution of the receptor