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目的:研究Noggin基因对骨肿瘤细胞MG63中Noggin,BMP-2,血管内皮生长因子(VEGF)及结缔组织生长因子(CTGF)的表达及细胞迁移侵袭的影响,探讨Noggin基因在骨肿瘤的发生和发展过程中的作用机制。方法:通过构建Noggin基因过表达重组腺病毒载体,并将其转染到骨肿瘤细胞MG63中,采用RT-PCR和Western blot分别检测空白对照组、过表达重组腺病毒组中Noggin,BMP-2,VEGF和CTGF的mRNA水平和蛋白水平,Transwell细胞迁移和侵袭能力实验考察两组细胞的迁移和侵袭能力。结果:成功构建Noggin基因过表达重组腺病毒载体,将其转染到骨肿瘤细胞MG63后,Noggin的mRNA及蛋白表达水平显著升高,BMP-2,VEGF及CTGF的mRNA及蛋白表达水平显著降低,转染Noggin基因过表达重组腺病毒的骨肿瘤细胞MG63迁移和侵袭的能力明显下降。结论:过表达Noggin基因能成功抑制骨肿瘤细胞MG63的侵袭和转移,有望成为骨肿瘤基因治疗的新方法。
Objective: To investigate the effect of Noggin gene on the expression of Noggin, BMP-2, VEGF and CTGF and the cell migration and invasion in the bone tumor cell MG63 and to explore the role of Noggin gene in the development of bone tumor and Mechanism of development in the process of action. Methods: Noggin gene was overexpressed by recombinant adenovirus vector and transfected into MG63 cells. The expression of Noggin and BMP-2 was detected by RT-PCR and Western blot respectively in blank control group and overexpression recombinant adenovirus group , The mRNA and protein levels of VEGF and CTGF, the migration and invasion ability of Transwell cells. Results: Noggin gene was overexpressed successfully and transfected into MG63 cells. The expression of Noggin mRNA and protein was significantly increased, and the mRNA and protein expressions of BMP-2, VEGF and CTGF were significantly decreased , The ability of MG63 cells to transfect and metastasize to bone tumor cells transfected with Noggin gene was significantly decreased. Conclusion: Overexpression of Noggin gene can successfully inhibit the invasion and metastasis of bone tumor cells MG63, which is expected to become a new method of gene therapy for bone tumors.