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目的 :研究Cl-通道在鼻咽癌细胞调节性容积回缩 (RVD)、增殖及细胞周期分布中的作用。方法 :活细胞图像分析低分化鼻咽癌细胞 (CNE - 2Z)RVD ,用台盼蓝拒染法检测细胞存活率 ,MTT法检测细胞增殖能力。用流式细胞仪测定细胞周期不同时相细胞百分率。结果 :Cl-通道抑制剂硝基苯丙胺基苯甲酸 (NPPB)剂量依赖性抑制RVD和细胞增殖 ,10 0 μmol/LNPPB明显阻抑细胞周期进程 ,使细胞停滞于G1期 ,G1期细胞百分率从 5 4 %提高到71% ,但对细胞存活率没有显著影响。结论 :阻抑Cl-通道可阻滞细胞于G1期而抑制细胞增殖。提示Cl-通道和RVD的激活是促进细胞从G1期进入S期和维持增殖所必需的因素。
Objective: To investigate the role of Cl-channel in regulating volumetric retraction (RVD), proliferation and cell cycle distribution of nasopharyngeal carcinoma cells. Methods: The survival rate of poorly differentiated nasopharyngeal carcinoma cells (CNE - 2Z) was analyzed by viable cell images. The viability of cells was detected by trypan blue exclusion. The proliferation of cells was detected by MTT assay. Flow cytometry was used to determine the cell percentage at different phases of the cell cycle. RESULTS: ClP channel inhibitor nitrophenyl aminobenzoic acid (NPPB) inhibited RVD and cell proliferation in a dose-dependent manner. 100 μmol / LNPPB significantly inhibited cell cycle progression and arrested cells in G1 phase. The percentage of cells in G1 phase increased from 5 4% to 71%, but had no significant effect on cell viability. Conclusion: Inhibition of Cl-channel can arrest cells in G1 phase and inhibit cell proliferation. It is suggested that the activation of Cl-channel and RVD is the necessary factor to promote the cell entering S phase from G1 phase and maintaining the proliferation.