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将感染性柏氏禽刺螨进行冰冻切片,以IFAT检测,结果首次证明革螨的肠组织及生殖器官(卵巢、及子宫孕卵)等部位的细胞内有流行性出血热病毒(EHFV)特异性荧光颗粒,而对照组则阴性。两个感染后的柏氏禽刺螨群体,一个是在第一若螨期感染的,另一个是感染成螨后,挑出其第2代若螨,均在适宜条件下饲养,不仅在饲养15天、25天叮刺乳鼠获阳性结果,而且在158天时将螨洗涤、研磨制成悬液接种乳鼠后,经IFAT检测鼠肺,也均获阳性结果。首次证明此螨群体能维持EHFV158天(约5个月)以上。以上的试验从螨体组织切片上和感染后进行繁殖的螨群体中证明EHFV的存在和维持,都说明此螨确系EHFV的适宜媒介和贮存宿主。
Frozen sections of infective Boehringer moths were detected by IFAT. The results showed that the specificities of epidemic hemorrhagic fever virus (EHFV) in the cells of the gut mite tissues such as the intestinal tissues and genital organs (ovary and uterus) Fluorescent particles, while the control group was negative. Two infected populations of B. poultry mites, one infected at the first raphus stage and the other at infestation with mites, picked out their second generation nymphs and were raised under appropriate conditions, not only in rearing 15 Day, 25-day sting stab suckling positive results, and in 158 days when the mites were washed, ground into suede mice after vaccination, IFAT test rat lung, also have positive results. It was first demonstrated that this mite population can sustain EHFV for more than 158 days (about 5 months). The above experiment proved the existence and maintenance of EHFV from mite body tissue sections and mites population after infection, both showed that this mite was the proper medium and storage host for EHFV.