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[Objective] To optimize the extracting conditions of ursolic acid from FRUCTUS CHAENOMELIS and determine its content, so as to provide scientific evidence for the development, application and quality control of ursolic acid. [Method] The extraction conditions of ursolic acid were optimized by orthogonal test, and content of ursolic acid was determined by HPLC. [Result] The optimal extraction conditions were as follows: extraction duration of 30 min, extraction solvent of absolute ethanol, ethanol dosage of 25.0 ml. Under the optimum conditions, the content of ursolic acid was 0.324%. Ursolic acid concentration had good linear relationship with peak area within the range of 0.650-3.250 μg/ml (r=0.999 1), and the mean recovery rate of ursolic acid was 99.20% with RSD of 0.30%. [Conclusion] The optimum extraction conditions were stable and efficient, and HPLC method was simple, sensitive and accurate. This study provided scientific evidence for the utilization and quality control of ursolic acid.
[Objective] To optimize the extracting conditions of ursolic acid from FRUCTUS CHAENOMELIS and determine its content, so as to provide scientific evidence for the development, application and quality control of ursolic acid. [Method] The extraction conditions of ursolic acid were optimized by orthogonal test, and content of ursolic acid was determined by HPLC. [Result] The optimal extraction conditions were as follows: extraction duration of 30 min, extraction solvent of absolute ethanol, ethanol dosage of 25.0 ml. Under the optimum conditions, the content of ursolic acid was 0.324%. Ursolic acid concentration had good linear relationship with peak area within the range of 0.650-3.250 μg / ml (r = 0.999 1), and the mean recovery rate of ursolic acid was 99.20% with RSD of 0.30% Conclusion] The optimum extraction conditions were stable and efficient, and HPLC method was simple, sensitive and accurate. This study provided scientific evidence for the utilization and quality control of urso lic acid.