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为了探讨单核细胞因子在激活人体中性粒细胞杀死福氏纳格勒阿米巴的作用,本文进行了下列三方面观察:1.条件培养基培养的中性粒细胞杀灭阿米巴的观察:11次试验结果表明有胎牛血清、正常兔血清或抗纳氏阿米巴血清时,中性粒细胞不能杀灭阿米巴。用条件培养基(以单核细胞2×10~6/ml孵育于含10%灭活的胎牛血清的RPMI 1640培养基,其中有或无1μg/ml PHA)培养的中性粒细胞加胎牛血清或正常兔血清时,也不能杀灭阿米巴;如有抗血清存在,则可杀死(p<0.001)。5次试验结果表明只有抗体时,阿
In order to investigate the role of monocytes in the activation of human neutrophils to kill F. amoebae, the following three aspects were observed: 1. Neutrophils cultured in conditioned media kill amoebae Observations: Neutrophils do not kill amoebae when tested in eleven trials showing that there is fetal calf serum, normal rabbit serum or anti-natamycin serum. Neutrophils incubated with conditioned media (2 × 10 ~ 6 / ml monocytes in RPMI 1640 medium with 10% inactivated fetal calf serum with or without 1 μg / ml PHA) Also, amoeba was not killed by bovine serum or normal rabbit serum; it was killed if antisera were present (p <0.001). Five times the test results show that only antibodies, Ah