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扩展青霉(P. expansum ) PF868 所产脂肪酶通过硫酸铵沉淀、DEAE-纤维素柱层析以及Sephacryl200 柱层析等步骤被纯化了74 倍, 最终比活力为69.7 u/m g.纯化后的脂肪酶经过SDS-聚丙烯酰胺电泳和微内径高效液相色谱仪检测分别达到了SDS-PAGE纯和微内径高效液相色谱纯. 分子量经SDS-PAGE确定为28.44 kD. 脂肪酶N-端氨基酸序列测定的结果是: ATADAAAFPD——这与其他一些真菌产的脂肪酶的序列具有较大的同源性.
The lipase produced by P. expansum PF868 was purified 74-fold by ammonium sulfate precipitation, DEAE-cellulose column chromatography and Sephacryl 200 column chromatography with a final specific activity of 69.7 u / m G. After purification SDS-polyacrylamide electrophoresis and micro-inner-diameter high-performance liquid chromatography were used to detect the purity of the lipase by SDS-PAGE and HPLC respectively. The molecular weight was determined to be 28.44 kD by SDS-PAGE. The result of the amino acid sequence determination is: ATADAAAFPD - This has a greater homology to the sequences of lipases produced by some other fungi.