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以“韩国甜椒”为试材,研究外植体种类、苗龄、基本培养基种类、激素组合等多种因素对辣椒组织再生的影响。结果表明:带柄子叶再生能力较子叶和下胚轴切段强,14d苗龄幼苗的外植体芽分化率较高,最佳芽分化培养基为MB+4.5mg/L BA+0.5mg/L IAA+4mg/L AgNO3+200mg/L Cef,最佳芽伸长培养基为MS+3mg/L BA+0.3mg/L IAA+2mg/L GA3+4mg/L AgNO3+200mg/L Cef,生根培养基先用1/2MS+0.4mg/L NAA+4mg/L AgNO3,继代用1/2MS+0.4mg/L NAA+0.2%活性炭。
Using “South Korea sweet pepper” as test material, the effects of explant types, seedling ages, basic medium types and hormone combinations on the tissue regeneration of pepper were studied. The results showed that the regeneration ability of cotyledons with cotyledon was stronger than that of cotyledon and hypocotyl cuttings. The bud differentiation rate of explants of 14-day-old seedlings was higher than that of cotyledons and hypocotyl cuttings. The optimal bud differentiation medium was MB + 4.5mg / L BA + 0.5mg / L IAA + 4mg / L AgNO3 + 200mg / L Cef, the best bud elongation medium was MS + 3mg / L BA + 0.3mg / L IAA + 2mg / L GA3 + 4mg / L AgNO3 + 200mg / The medium was first replaced with 1/2 MS + 0.4 mg / L NAA + 4 mg / L AgNO 3 and 1/2 MS + 0.4 mg / L NAA + 0.2% activated charcoal.