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目的观察汉丹肝乐对大鼠贮脂细胞增殖的影响 ,探讨其抗肝纤维化作用机理。方法用链蛋白酶和胶原酶原位灌注 ,Nycodenz密度梯度离心分离大鼠肝贮脂细胞 ,并以四甲基偶氮唑蓝(MTT)比色法观察汉丹肝乐对贮脂细胞增殖效应。结果汉丹肝乐可影响贮脂细胞的增殖 ,其浓度在180～ 72 0 μg ml时对贮脂细胞有抑制作用 (P <0 0 5 ) ,浓度≤ 90 μg ml时对贮脂细胞增殖无明显抑制作用 (P <0 0 5 )。结论汉丹肝乐发挥抗肝纤维化作用 ,作用机理之一是抑制贮脂细胞增殖
Objective To observe the effect of Han Dan Gagaku on the proliferation of fat-storing cells in rats, and to explore its mechanism of anti-hepatic fibrosis. Methods Rat hepatic steatosis cells were isolated by in situ perfusion with streptavidin and collagenase and centrifuged by Nycodenz density gradient centrifugation. MTT assay was used to observe the effect of Hantan Dan Le on the proliferation of osteoporotic cells. Results Han Dan Gensile could affect the proliferation of lipid-storing cells. When the concentration of Dan-Dan-Gan was 180 ~ 72 0 μg ml, the proliferation of fat-storing cells was inhibited (P <0 05) Significant inhibition (P <0 05). Conclusion Han Dan Geng play anti-liver fibrosis, one of the mechanisms of inhibition of proliferation of fat storage cells