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用从桑叶中分离提取的粗多糖MLPⅡ连续5周灌胃给药治疗Ⅱ型糖尿病模型大鼠,在电子显微镜下观察大鼠胰岛β细胞超微结构,采用免疫组化法检测核转录因子κB(nuclear transcription factorκB,NF-κB)的表达变化,并利用Western blot、半定量RT-PCR法分别检测肿瘤坏死因子α(tumor necrosis factorα,TNF-α)和胰岛素2(insulin 2,INS-2)在蛋白质表达水平和基因转录水平的变化。与糖尿病模型对照组大鼠相比,桑叶多糖MLPⅡ治疗组糖尿病模型大鼠的胰岛β细胞超微结构损伤程度明显减轻,胰岛β细胞中NF-κB的表达明显降低,胰腺组织中TNF-α蛋白的表达和基因mRNA的转录显著下调(P<0.01),而INS-2蛋白的表达和基因mRNA的转录显著上调(P<0.01)。试验结果表明,桑叶多糖MLPⅡ可明显改善Ⅱ型糖尿病模型大鼠胰腺组织氧化应激损伤,其作用机制可能与下调胰腺组织NF-κB和TNF-α的表达有关。
Using the polysaccharides MLP Ⅱ isolated and extracted from mulberry leaves for 5 consecutive weeks, the type Ⅱ diabetic rats were treated by intragastric administration, and the ultrastructures of pancreatic β cells were observed under the electron microscope. The expression of nuclear factor kappa B The expression of NF-κB was detected by Western blot and semi-quantitative RT-PCR. The levels of tumor necrosis factorα (TNF-α) and insulin-2 (INS-2) Changes in protein expression and gene transcription level. Compared with diabetic model control group, mulberry leaf polysaccharide MLPⅡ treatment group diabetic rat model of pancreatic β-cell ultrastructure damage was significantly reduced, the expression of NF-κB in pancreatic β cells was significantly decreased, the level of TNF-α in pancreatic tissue Protein expression and gene mRNA transcription were significantly down-regulated (P <0.01), while INS-2 protein expression and mRNA transcription were significantly up-regulated (P <0.01). The results showed that mulberry leaf polysaccharide MLP Ⅱ can significantly improve the oxidative stress injury in the pancreas of type Ⅱ diabetic rats, and its mechanism may be related to the down-regulation of the expression of NF-κB and TNF-α in the pancreas.