本文介绍一种简单、价廉、可自小量血或大量血中获得相当纯的单核细胞的方法,此方法是基于血浆加氯化钠变成高渗经短时间培育后单核细胞发生改变的原理。在一定限度内,这样处理使淋巴细胞由于失水而比重加大,单核细胞由于强烈的吞饮作用,比重改变很小。本文略述利用这种现象连同稍加改进的B(?)yum技术密度梯度分离以获得单核细胞悬液的最简单方法。 This article presents a simple, inexpensive method for obtaining relatively pure monocytes from a small amount of blood or a large amount of blood based on the mononuclear cell that occurs when plasma plus sodium chloride becomes hypertonic and incubated for a short period of time Change the principle. Within a certain limit, this treatment causes lymphocytes to increase their proportion due to dehydration. Monocytes, due to their intensive phagocytosis, show a small change in specific gravity. This article outlines the easiest way to obtain monocyte suspensions by using this phenomenon along with a slight improvement in density gradient B (?) Yum technique.