论文部分内容阅读
随着国家载人航天项目“神舟四号”飞船的成功发射和顺利返回,其携带的植物细胞——烟草(黄花品种)叶内原生质体与烟草(革新一号)叶肉细胞脱液泡原生质体空间电融合实验获得成功,细胞核染色的结果显示,空间飞行样品中双核细胞的比率为18.8％,多核细胞的比率为2.1％,分别比地面对照样品(1.65％和0.34)增加10.4和5.2倍.细胞活力为53.1%,地面对照细胞的活力为38.0％,相对提高了39.7％。同时,微重力环境中融合细胞的代谢活动也发生了显著的改变。此次实验的成功表明空间微重力环境是改善细胞电融合技术的重要途径。
With the successful launching and successful return of the spacecraft “Shenzhou IV” of the national manned space project, the protoplasts in the leaves of tobacco (yellow variety) and the mesophyll decellularized protoplast space of tobacco The results of nucleus staining showed that the percentage of binucleated cells in space flight samples was 18.8% and that of multinucleated cells was 2.1%, which increased by 10.4 and 5.2 times respectively compared with the ground control samples (1.65% and 0.34) The viability was 53.1%, the activity of ground control cells was 38.0%, a relative increase of 39.7%. At the same time, the metabolic activity of fused cells in the microgravity environment has also undergone significant changes. The success of this experiment shows that space micro-gravity environment is an important way to improve cell electrofusion technology.