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应用免疫荧光组织化学三标方法结合激光共聚焦显微镜技术研究了辣椒素受体(VR1)在大鼠舌咽神经和迷走神经内脏感觉神经节结状神经节(NG)和岩神经节(PG)内的表达,以及与降钙素基因相关肽(CGRP)、植物凝集素(IB4)的共存。结果显示:VR1在NG和PG内中、小型神经元胞体和神经纤维存在广泛的表达。许多VR1阳性神经元呈IB4阳性或与CGRP共存。在NG,CGRP阳性神经元数量较少,约有71.4%±3.8%VR1阳性神经元与IB4共存,只有7.1%±1.2%VR1阳性细胞与CGRP共存。PG内CGRP阳性神经元胞体数量较多,有55.7%±3.1%VR1阳性神经元与IB4共存;有38.7%±2.7%VR1阳性细胞同时呈CGRP阳性。两个神经节内IB4/CGRP双标神经元或VR1/CGRP/IB4三标神经元数量稀少。上述结果提示舌咽神经和迷走神经内脏感觉神经节内存在VR1/IB4和VR1/CGRP两种不同的与伤害性刺激相关的VR1阳性神经元亚群。
The expression of capsaicin receptor (VR1) in rat glossopharyngeal and vagal visceral sensory ganglion nodules (NG) and petrosal ganglia (PG) were studied by immunofluorescence histochemistry and laser scanning confocal microscopy , As well as the coexistence of calcitonin gene-related peptide (CGRP) and lectin (IB4). The results showed that VR1 was widely expressed in small neuronal somatic cells and nerve fibers in both NG and PG. Many VR1-positive neurons are IB4-positive or coexist with CGRP. In NG, the number of CGRP positive neurons was less, and about 71.4% ± 3.8% of VR1 positive neurons coexisted with IB4. Only 7.1% ± 1.2% of VR1 positive cells coexisted with CGRP. The number of CGRP-positive neurons was significantly higher in PG, with 55.7% ± 3.1% VR1-positive neurons coexisting with IB4. 38.7% ± 2.7% of VR1-positive cells were positive for CGRP at the same time. IB4 / CGRP double-labeled neurons or VR1 / CGRP / IB4 triple-labeled neurons were sparse in both ganglia. The above results suggest that VR1 / IB4 and VR1 / CGRP are two subtypes of VR1-positive neurons that are involved in noxious stimulation in glossopharyngeal and vagal visceral sensory ganglia.