【摘 要】
：
Human μ-opioid receptor (HμOR) with a tag of six consecutive hisfidines at its carboxyl terminus was overexpressed in recombinant baculovirus infected Sf9 in
【出 处】
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Acta Pharmacologica Sinica
论文部分内容阅读
Human μ-opioid receptor (HμOR) with a tag of six consecutive hisfidines at its carboxyl terminus was overexpressed in recombinant baculovirus infected Sf9 insect cells.The recombinant HμOR had high affinity to[~3H] diprenorphine and[~3H]ohmefentanyl (Ohm), and the binding was strongly inhibited by μ-selective agonists DAGO, Ohm, and morphine, but neither
Human μ-opioid receptor (HμOR) with a tag of six consecutive hisfidines at its carboxyl terminus was overexpressed in recombinant baculovirus infected Sf9 insect cells. The recombinant HμOR had high affinity to [~ 3H] diprenorphine and [~ 3H] ohmefentanyl (Ohm) , and the binding was strongly inhibited by μ-selective agonists DAGO, Ohm, and morphine, but neither
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