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背景:万古霉素玻璃体内注射后的药代动力学资料较少。目的:观察万古霉素玻璃体腔内注射后在清醒兔眼玻璃体内的浓度。方法:将微透析探针植入清醒正常兔眼和细菌性眼内炎兔眼玻璃体内24h后,向玻璃体内注射10g/L万古霉素0.1mL,利用微透析采样技术联合高效液相色谱法连续检测万古霉素注射后0.5,1,2,4,6,12,24,48,72,84h,兔眼玻璃体内的药物浓度。结果与结论:万古霉素在正常兔眼玻璃体内的代谢呈开放式二室模型,玻璃体内的峰值浓度为695.92mg/L,半衰期51.66h;在细菌性眼内炎兔眼玻璃体内的代谢呈一室模型,万古霉素的峰值浓度为713.35mg/L,半衰期为11.91h。所有动物给药84h,玻璃体内万古霉素的浓度均高于最小抑菌浓度。实验在动物清醒状态下实时、连续、动态采样,检测结果准确,能满足万古霉素药动学分析的需要。
Background: Pharmacokinetic data after vancomycin vitreous injections are less. Objective: To observe the intravitreal concentration of vancomycin in vitreous cavity after intravitreal injection. Methods: The microdialysis probe was implanted into the vitreous of rabbits with normal eye and bacterial endophthalmitis for 24 hours. Then 0.1 mL of vancomycin 10 g / L was injected into the vitreous. The microdialysis probes were combined with high performance liquid chromatography 0.5, 1, 2, 4, 6, 12, 24, 48, 72, 84 h after injection of vancomycin were continuously detected in vitreous body of rabbits. RESULTS AND CONCLUSION: The metabolism of vancomycin in the vitreous of normal rabbits was an open two-compartment model. The intravitreal peak concentration was 695.92mg / L and the half-life was 51.66h. The vitreous metabolism in rabbit eyes with bacterial endophthalmitis was In the one-compartment model, vancomycin had a peak concentration of 713.35 mg / L and a half-life of 11.91 h. All animals were administered 84h, vitreous vancomycin concentrations were higher than the minimum inhibitory concentration. Experimental real-time, continuous, dynamic sampling in the awake state of the animal, the test results are accurate and can meet the needs of vancomycin pharmacokinetic analysis.