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目的探讨人类白细胞抗原(HLA)DRB1和DQB1位点基因多态性与尘肺发病的关系。方法采用1∶1配比的病例-对照研究方法。尘肺病例组为113名Ⅰ期尘肺患者,对照组为与尘肺病例组年龄相近、同性别、同民族、同一工作地点、开始接尘时间和累积接尘工龄相差不超过2年的接尘工人。用聚合酶链反应序列特异性引物分析方法检测HLADRB1和DQB1共9个位点的等位基因。用SAS6.12软件进行单因素和多因素分析。结果尘肺病例组的HLADRB108等位基因频率高于对照组,差异有统计学意义(P<0.01),OR值为6.000,95%CI1.906～18.941;HLADRB109、HLADQB106等位基因频率低于对照组,差异有统计学意义(P<0.01),OR值为0.259、0.300,95%CI为0.115～0.584、0.144～0.627;1∶1条件Logistic回归分析表明,HLADRB108、HLADRB109和HLADQB106等位基因频率与尘肺有关联,调整OR值分别为7.804、0.225和0.269,95%CI分别为2.077～29.307、0.083～0.609和0.117～0.613。生存分析表明,HLADRB108等位基因为尘肺潜伏期的危险因素;HLADQB106为保护因素。结论HLADRB108等位基因可能为尘肺易感的危险因素,HLADQB106可能是抗尘肺的保护性的等位基因。
Objective To investigate the relationship between human leukocyte antigen (HLA) DRB1 and DQB1 gene polymorphisms and the incidence of pneumoconiosis. Methods A 1: 1 matched case-control study was used. The pneumoconiosis patients group consisted of 113 patients with stage I pneumoconiosis, while the control group patients with dust pneumoconiosis who were close to the same age, same sex, same ethnicity, same place of work, time of starting dust collection and accumulated dust service were less than 2 years. Nine alleles of HLADRB1 and DQB1 were detected by polymerase chain reaction sequence-specific primer analysis. Using SAS6.12 software for single factor and multivariate analysis. Results The frequency of HLADRB108 alleles in patients with pneumoconiosis was significantly higher than that in controls (P <0.01), OR was 6.000 and 95% CI of 1.906 to 18.941, and the frequency of HLADRB109 and HLADQB106 alleles was lower than that of controls , The difference was statistically significant (P <0.01), OR was 0.259,0.300, 95% CI was 0.115 ~ 0.584,0.144 ~ 0.627; 1: 1 conditional Logistic regression analysis showed that HLADRB108, HLADRB109 and HLADQB106 allele frequency and Pneumoconiosis was associated with adjusted OR values of 7.804, 0.225 and 0.269, respectively. The 95% CI was 2.077-29.307,0.083-0.609 and 0.117-0.613, respectively. Survival analysis showed that HLADRB108 allele was a risk factor for pneumoconiosis and HLADQB106 was a protective factor. Conclusion HLADRB108 allele may be a risk factor for susceptibility to pneumoconiosis. HLADQB106 may be a protective allele for pneumoconiosis.