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目的 探讨 P2 1 waf1和 Cyclin E在肺鳞癌组织中表达。方法 应用细胞周期调控因子 P2 1 waf1、 Cyclin E单克隆抗体 ,对 5 4例肺鳞状细胞癌的纤支镜活检标本和 10例正常肺组织进行免疫组化染色研究。结果 在肺鳞癌组织中 P2 1 waf1 呈低水平表达 ,其阳性表达率 (11/ 5 4)仅为 2 0 .4%。在肺鳞癌组织中 Cyclin E阳性表达率 (17/ 5 4)为 31.5 %。低分化及有淋巴结转移的病例 ,其 Cyclin E表达量明显高于高中分化及无淋巴结转移的病例。结论 P2 1 waf1在肺鳞癌组织中呈低表达 ,Cyclin E表达可作为判断肺鳞癌恶性程度的一个指标
Objective To investigate the expression of P2 1 waf1 and Cyclin E in squamous cell lung cancer. Methods Immunofluorescence staining was performed on 54 biopsy specimens of lung squamous cell carcinoma and 10 normal lung tissues using monoclonal antibody against cell cycle regulator P2 1 waf1 and Cyclin E. Results The expression of P2 1 waf1 in lung squamous cell carcinoma tissues was low, and the positive expression rate (11/54) was only 20.4%. The positive expression rate of Cyclin E in squamous cell carcinoma of the lung (17/54) was 31.5%. The poorly differentiated and lymph node metastasis cases, the expression of Cyclin E was significantly higher than the high school differentiation and no cases of lymph node metastasis. Conclusion P2 1 waf1 is low expressed in squamous cell carcinoma of the lung, and the expression of Cyclin E may be used as an indicator of the malignancy of squamous cell carcinoma of the lung