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长春胺(vincamine)是小蔓长春花(Vinca minor)叶子和茎中的主要生物碱。临床研究证明,长春胺对脑循环不全引起的各种疾病有治疗作用,对脑血管病引起的各种功能障碍有改善作用。为对小蔓长春花的栽培条件进行研究以及对长春胺提取工艺方法的考察,作者选用薄层法进行分离,分离后的长春胺斑点可用直接法(即光密度计法)或洗脱法(即紫外分光光度法)测定含量;光密度计法的线性范围为0.5～3.0μg/点,纯品的变异系数为2.02%(n=9)。方法快速、灵敏,适合进行大批样
Vincamine is the main alkaloid in the leaves and stems of Vinca minor. Clinical studies have demonstrated that vincamine has a therapeutic effect on various diseases caused by cerebral insufficiency, and it has an improved effect on various functional disorders caused by cerebrovascular disease. In order to study the cultivation conditions of Vinca minor, and to study the extraction process of Vinca alamine, the authors used a thin layer method for separation. The isolated vincamine spots can be directly measured (ie, densitometry) or eluted ( That is, UV spectrophotometry was used to determine the content; the linear range of densitometry was 0.5-3.0 μg/point, and the variation coefficient of pure product was 2.02% (n=9). The method is fast, sensitive and suitable for a large number of samples