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目的比较大蒜多糖与硒化大蒜多糖对经过氧化氢(H2O2)诱导损伤的大鼠嗜铬瘤细胞株(PC12)的保护作用。方法用H2O2建立PC12细胞损伤模型,应用四氮唑蓝(MTT)比色法检测PC12细胞的活力;化学比色法测定细胞培养液中乳酸脱氢酶(LDH)释放量、细胞内和细胞培养液中丙二醛(MDA)含量及超氧化物歧化酶(SOD)的活性。结果硒化大蒜多糖、大蒜多糖500μg/ml及以上剂量组和亚硒酸钠12.50μg/ml及以上剂量组均能有效抑制由H2O2引起的细胞存活率的下降(P<0.01)。硒化大蒜多糖、大蒜多糖2000μg/ml可显著降低LDH释放量和细胞培养液及细胞内的MDA含量,以及提高SOD活性(P<0.01),表现出良好的抗氧化活性,而硒化大蒜多糖的抗氧化作用更为明显(P<0.05)。结论硒化大蒜多糖对经H2O2诱导损伤的PC12细胞具有保护作用,这种保护作用明显优于大蒜多糖或单纯硒,其作用机制可能与提高PC12细胞的抗氧化能力有关。
Objective To compare the protective effects of garlic polysaccharide and selenium garlic polysaccharide on rat pheochromocytoma cell line (PC12) induced by hydrogen peroxide (H2O2). Methods PC12 cell injury model was established by H2O2. The viability of PC12 cells was detected by MTT assay. The release of lactate dehydrogenase (LDH) in cell culture medium was measured by chemical colorimetry. The intracellular and cell culture Fluid MDA content and superoxide dismutase (SOD) activity. Results The selenated garlic polysaccharide, garlic polysaccharide 500μg / ml and above dose group and sodium selenite 12.50μg / ml and above dose group can effectively inhibit the decrease of cell viability induced by H2O2 (P <0.01). Selenium garlic polysaccharide, garlic polysaccharide 2000μg / ml can significantly reduce the amount of LDH release and cell culture medium and intracellular MDA content, and improve SOD activity (P <0.01), showing good antioxidant activity, and selenium garlic polysaccharide The antioxidant effect was more obvious (P <0.05). Conclusion Selenium-enriched garlic polysaccharide has the protective effect on PC12 cells induced by H2O2, and its protective effect is obviously better than that of garlic polysaccharide or selenium alone. The mechanism may be related to the anti-oxidation ability of PC12 cells.