论文部分内容阅读
目的研究内质网应激相关蛋白CHOP/GADD153在胎膜早破中的表达及其意义。方法选择胎膜早破患者40例(早破组)及相应孕周择期剖宫产孕妇40例(对照组),采用RT-PCR技术和Western blot法检测两组孕妇胎膜组织中CHOP/GADD153的mRNA与蛋白表达。结果早破组胎膜组织中CHOP/GADD153的mRNA[(8.016±0.016)vs.(1.716±0.251)]与蛋白表达水平[(15.210±0.217)vs.(2.710±0.019)]均显著高于对照组(P<0.05)。结论 CHOP/GADD153表达的增高与胎膜早破的发病相关。
Objective To study the expression of endoplasmic reticulum stress related protein CHOP / GADD153 in premature rupture of membranes and its significance. Methods 40 cases of premature rupture of membranes (premature rupture group) and 40 gestational weeks elective cesarean section pregnant women (control group) were selected. The expression of CHOP / GADD153 in fetal membranes was detected by RT-PCR and Western blot. MRNA and protein expression. Results The mRNA level of CHOP / GADD153 in CHOP / GADD153 group was significantly higher than that in control group ([(8.016 ± 0.016) vs. (1.716 ± 0.251)] and protein expression level [(15.210 ± 0.217) vs. (2.710 ± 0.019) Group (P <0.05). Conclusion CHOP / GADD153 expression is associated with the incidence of premature rupture of membranes.