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目的:建立石韦药材的HPLC指纹图谱,为其全面质量评价和控制提供参考。方法:采用HPLC法,色谱条件:Diamonsil C18柱(250 mm×4.6 mm,5μm),以乙腈-0.8%乙酸水溶液梯度洗脱,测定了44批石韦样品的指纹图谱,建立了石韦与有柄石韦指纹图谱的共有模式以及庐山石韦的共有模式,应用相似度分析、聚类分析和主成分分析对其进行分析评价。结果:中国药典品种中,石韦与有柄石韦指纹图谱相似,但与庐山石韦有较大差异;相似石韦与光石韦与中国药典品种区别显著。结论:本方法可简便、快速地对石韦药材进行比较分析,所建立的指纹图谱为原料药材的选购和石韦品种的鉴别提供实验依据。
OBJECTIVE: To establish the HPLC fingerprints of Shiwei medicinal materials and provide a reference for its overall quality evaluation and control. Methods: The chromatographic conditions were as follows: Diamonsil C18 column (250 mm × 4.6 mm, 5 μm), gradient elution with acetonitrile-0.8% aqueous acetic acid. The fingerprint of 44 samples of Shigella was determined. The common mode of stony fingerprint and the common mode of Lushan Shiwei were analyzed and evaluated by similarity analysis, cluster analysis and principal component analysis. Results: Among the Chinese Pharmacopoeia varieties, Shiwei was similar to that of Shanyi Shiwei, but there was a big difference with Shiwei in Lushan Mountain. The difference between Shiwei and Guangfuwei and that of Chinese Pharmacopoeia was significant. Conclusion: This method can be simple and rapid comparative analysis of Shi Wei herbs, the fingerprint established for the purchase of raw materials and identification of Shi Wei breed provide experimental evidence.