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目的探究栀子柏皮汤合煎与分煎提取液中有效成分栀子苷、西红花苷-Ⅰ、西红花苷-Ⅱ、盐酸药根碱、盐酸巴马汀、盐酸小檗碱、甘草苷和甘草酸的变化。方法采用Agilent TC-C18(4.6mm×250mm,5μm)色谱柱,流动相以乙腈-0.2%磷酸水溶液,分别在238nm波长下检测栀子苷、甘草苷和甘草酸,在265nm波长下检测盐酸药根碱、盐酸巴马汀和盐酸小檗碱,在440nm波长下检测西红花苷-Ι和西红花苷-ΙΙ;流速为0.8 ml/min,梯度洗脱,柱温30℃。结果合煎剂中总生物碱、甘草酸的含量低于分煎剂;西红花苷类化合物(西红花苷-I、西红花苷-ΙΙ)的含量明显高于分煎剂;合煎剂中栀子苷、甘草苷与分煎剂中含量无明显变化。结论栀子柏皮汤合煎剂中有效成分溶出增加、毒性成分含量降低,比分煎剂质量优越。
Objective To explore the effects of gardenia berberine, crocin-Ⅰ, crocin-Ⅱ, jatrorrhizine hydrochloride, palmatine hydrochloride, berberine hydrochloride, Changes in glycyrrhizin and glycyrrhizin. Methods Geniposide, glycyrrhizin and glycyrrhizin were detected by Agilent TC-C18 column (4.6 mm × 250 mm, 5 μm) with mobile phase of acetonitrile-0.2% phosphoric acid at 238 nm. Root base, palmatine hydrochloride and berberine hydrochloride, crocinil-1 and crocin-II were detected at a wavelength of 440 nm; the flow rate was 0.8 ml / min and the gradient was eluted at a column temperature of 30 ° C. The results of combined decoction of total alkaloids, glycyrrhizic acid content was lower than the decoction; crocin (caryol-I, crocin-II) was significantly higher than the decoction; In the decoction of geniposide, glycyrrhizin and decoction in the content of no significant change. Conclusion Fructus Gardeniae Bupi Decoction decoction increased the dissolution of active ingredients, reducing the content of toxic components, the ratio of decoction of superior quality.