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取雄性Wistar大鼠,随机分为4组,采用非气管暴露法分别注入15 mg/ml、30 mg/ml、60 mg/ml粉尘悬浮液和生理盐水各1 ml,每组7只大鼠分别在第1、3、7、14、21、28天6个时间点处死。离体进行支气管肺泡灌洗(BAL),BALF离心取上清液,然后采用酶联免疫吸附(ELISA)测定上清液中TNF-α、TGF-β含量。不同剂量的粉尘对TNF-α、TGF-β有显著影响(P<0.05),各剂量组在各时点TNF-α含量较对照组均有升高,除21 d高剂量组外差异均有统计学意义(P<0.05);TGF-β含量较对照组差异有统计学意义(P<0.05)。提示矽尘所致的纤维化早期TNF-α、TGF-β含量变化规律明显,抑制一种或两种细胞因子可有效阻滞纤维化的发生发展。
Male Wistar rats were randomly divided into 4 groups. Each group was injected with 1 ml of 15 mg / ml, 30 mg / ml, 60 mg / ml dust suspension and normal saline respectively by non-tracheal exposure method. On the 1st, 3rd, 7th, 14th, 21st and 28th day, 6 time points were sacrificed. Bronchoalveolar lavage (BAL) was performed in vitro, and the supernatant was harvested by centrifugation in BALF. The contents of TNF-α and TGF-β in the supernatant were determined by enzyme-linked immunosorbent assay (ELISA). The different doses of dust had significant effects on TNF-αand TGF-β (P <0.05). The TNF-α content of each dose group was higher than that of the control group at all time points, except the 21-day high dose group Statistical significance (P <0.05); The content of TGF-β was significantly different from the control group (P <0.05). It is suggested that the changes of TNF-α and TGF-β in the early stage of fibrosis induced by silica dust are obvious, and inhibition of one or two cytokines can effectively block the development of fibrosis.