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目的:建立山东道地产区金银花的快速高分离度液相色谱(RRLC)指纹图谱,为金银花的质量控制提供快速、科学的方法。方法:用Agilent Zorbax Eclipse XDB-C18(50mm×2.1mm,1.8μm)色谱柱,以乙腈(含0.1%甲酸)-0.1%甲酸水溶液为流动相梯度洗脱,检测波长238nm,柱温50℃,流速0.3m L/min,进样量2μL。结果:在20min内得到金银花药材的RRLC指纹图谱;8批样品中共获得31个共有特征色谱峰。结论:该方法快速、简便、准确、重复性好,为山东道地产区金银花的质量控制提供了科学依据。
OBJECTIVE: To establish a rapid high-resolution liquid chromatography (HPLC) fingerprint of honeysuckle in Shandong Road area, providing a fast and scientific method for the quality control of honeysuckle. METHODS: The mobile phase consisted of acetonitrile (containing 0.1% formic acid) and 0.1% formic acid as mobile phase with Agilent Zorbax Eclipse XDB-C18 column (50 mm × 2.1 mm, 1.8 μm) Flow rate 0.3m L / min, injection volume 2μL. Results: RRLC fingerprints of honeysuckle were obtained within 20min. A total of 31 common chromatographic peaks were obtained in 8 batches of samples. Conclusion: The method is rapid, simple, accurate, reproducible and provides a scientific basis for the quality control of honeysuckle in the real estate of Shandong Province.