目的 :从转录因子水平观察山莨菪碱能否减轻ＴＮＦ -α所致内皮细胞的凋亡 ,并探讨其机制是否与其抑制ＮＦ -κＢ活化有关。方法 :培养小牛肺动脉内皮细胞 (ＢＰＡＥＣ) ,将山莨菪碱 ( 0 2ｍｇ/ｍＬ)加入细胞培养基中 ,30ｍｉｎ后再加入ＴＮＦ -α( 2 50 0Ｕ/ｍＬ)损伤 2 4ｈ OBJECTIVE: To observe whether anisodamine can reduce the apoptosis of endothelial cells induced by TNF-α from the level of transcription factors and explore whether its mechanism is related to the inhibition of NF-κB activation. Methods: Bovine pulmonary artery endothelial cells (BPAECs) were cultured, and anisodamine (0 2 mg / mL) was added to the cell culture medium. After 30 min, TNF-α (2 50 0 U / mL)