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小麦-中间偃麦草易位系YW642含有一个源于中间偃麦草7X染色体的抗性基因Bdv2,对大麦黄矮病毒GAV株系具有高度抗性。为有效控制该病毒和阐明抗黄矮病机制,采用半定量RT-PCR的方法,研究了大麦黄矮病毒GAV株系在YW642及其感病姊妹系YW641中积累浓度的差异。分别在接种病毒不同时间、不同部位上取样,用半定量RT-PCR的方法来检测GAV的积累浓度。在接种部位,抗病植株中病毒的浓度远远低于感病植株。在侵染的前5d,抗病植株YW642中病毒会有一定程度的复制和积累,但随后病毒浓度开始下降,接种14~16d时没有检测到病毒;而在感病株系中,病毒积累的浓度远远高于抗病植株,并一直维持一个较高的浓度。在未接种部位,感病植株中可检测到较高浓度的病毒,说明病毒能从接种点很快运动到未接种部位,并大量复制。而在抗病系YW642中,未接种部位始终未检测到病毒。实验结果从分子水平上证明,在抗病植株中BYDV的复制和运动均受到了极大的抑制。这是抗病基因Bdv2与BYDV互作后,激活了一系列防御基因的结果。另外还确定了防御基因诱导表达的时间,为从抗病植株中分离抗病相关基因、研究抗黄矮病机制提供了取样的依据。
The wheat-Thinopyrum intermedium translocation line YW642 contains a resistance gene Bdv2 derived from the 7X chromosome of Thinopyrum intermedium and is highly resistant to the barley yellow dwarf virus GAV strain. In order to effectively control the virus and elucidate the mechanism of resistance to yellowish dwarf virus, the difference of accumulation concentration of the barley yellow dwarf virus GAV strain in YW642 and its susceptible sister strain YW641 was studied by semi-quantitative RT-PCR. Samples were collected at different times and at different sites of virus inoculation, and the concentration of GAV was detected by semi-quantitative RT-PCR. At the inoculation site, the concentration of virus in the resistant plants is much lower than that of the susceptible plants. In the first 5 days of infection, the virus in the resistant plant YW642 could be copied and accumulated to some extent, but then the virus concentration began to decline. No virus was detected at 14-16 days after inoculation. In the diseased plants, the virus accumulated The concentration is much higher than that of the resistant plants and has been maintained at a higher concentration. In the uninoculated sites, a higher concentration of virus could be detected in the susceptible plants, indicating that the virus could rapidly move from the inoculation site to the non-inoculated site and replicate in large quantities. In the resistant line YW642, the virus was not detected at the unvaccinated site. The experimental results show that BYDV replication and exercise are greatly inhibited in the resistant plants. This is the result of a series of defense genes activated by the interaction of the disease resistance gene Bdv2 and BYDV. In addition, the time for induced expression of defensive genes was also determined, which provided the basis for sampling for isolating disease-related genes from resistant plants and studying the mechanism of anti-yellowish dwarfism.