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禾本科山羊草属具有多种农田杂草,其中具节山羊草和节节麦是我国禁止进境的检疫性杂草,我国检验检疫部门在进口粮谷检疫中经常会截获具节山羊草和节节麦。由于检疫性山羊草属杂草在植株及种子形态上与其近似种不容易区分,给检疫鉴定带来了很大困难。本文研究了一种PCR-RFLP分子鉴定方法,能够快速、准确地将检疫性山羊草属杂草与非检疫性种类区分开来,解决了该类杂草鉴定难题。
Gramineae genus Aegilops have a variety of farmland weeds, of which section of the Aegilops and Aegilops ferry is our country banned the entry of quarantine weeds inspection and quarantine departments in the import grain and grain quarantine often seized a section of goat grass and Festival wheat. Quarantine of the weeds because of the quarantine plants and seeds in the form of similar species is not easy to distinguish, to the quarantine identification has brought great difficulties. In this paper, a molecular identification method of PCR-RFLP was studied, which can distinguish the quarantine Aegilops species from the non-quarantine species quickly and accurately, and solve the problem of the identification of this kind of weeds.