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对影响棉花花药愈伤组织诱导的因素进行了研究，结果表明：灭菌方法以０．１％的氯化汞消毒１０分钟灭菌较为彻底；培养方式以花药固体培养较易诱导出愈伤组织。不同基因型的诱导效应不同，亚洲棉较易，陆地棉次之，海岛棉较差，野生棉最难，陆地棉不同品种间也存在着差别。激素种类和浓度影响着棉花花药愈伤组织的诱导率和生长量，ＴＤＺ有利于愈伤组织的诱导，ＢＡ不利于愈伤组织的诱导；ＭＳ＋０．１ｍｇ／ＬＢＡ＋５．０ｍｇ／Ｌ２，４－Ｄ是诱导棉花花药脱分化形成愈伤组织的较好培养基；ＭＳ＋０．１ｍｇ／ＬＢＡ＋０．１ｍｇ／Ｌ２，４－Ｄ或ＭＳ＋１．０ｍｇ／ＬＢＡ＋１．０ｍｇ／Ｌ２，４－Ｄ最有利于棉花花药愈伤组织的生长和增殖。
The factors influencing the induction of cotton anther callus were studied. The results showed that the sterilization method was completely sterilized with 0.1% mercuric chloride for 10 minutes, and the culture was easier to induce the callus by anther solid culture . The induction effect of different genotypes is different, Asian cotton easier, followed by upland cotton, island cotton worse, wild cotton is the hardest, there are differences between different varieties of upland cotton. The type and concentration of hormones affected the induction rate and growth of anther callus in cotton, TDZ was conducive to callus induction, BA was not conducive to callus induction; MS + 0.1mg / LBA + 5.0mg / L2,4-D Inducing cotton anther dedifferentiation to form callus; MS + 0.1mg / LBA + 0.1mg / L2,4-D or MS + 1.0mg / LBA + 1.0mg / L2,4-D was the most conducive to cotton anther callus Growth and proliferation.