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目的探讨蛋白质磷酸化酶抑制剂对肺癌细胞株的增殖抑制作用。方法用蛋白质磷酸化酶抑制剂对６株小细胞肺癌和７株非小细胞肺癌细胞株的增殖抑制效果进行了检测，并采用免疫法，测定了蛋白质磷酸化酶抑制剂对肺癌细胞内Ｒｂ蛋白表达的影响。结果随其浓度的增加，对肺癌细胞株的增殖抑制作用增强，小细胞肺癌的半数抑制量平均值ＳＴ为９５ｎＭ，ＵＣＮ－０１为５８８ｎＭ，非小细胞肺癌则分别为１８８ｎＭ和１０２９ｎＭ，ＳＴ和ＵＣＮ－０１可抑制肺癌细胞Ｒｂ蛋白的磷酸化。结论蛋白质磷酸化酶抑制剂通过抑制Ｒｂ蛋白的磷酸化，从而抑制肺癌细胞的增殖
Objective To investigate the inhibitory effect of protein phosphorylase inhibitor on the proliferation of lung cancer cell lines. Methods The inhibitory effects of protein phosphatase inhibitors on the proliferation of 6 small cell lung cancer and 7 non-small cell lung cancer cell lines were detected. The protein phosphatase inhibitor was used to determine the Rb protein in lung cancer cells by immunoassay. The effect of expression. Results As the concentration increased, the inhibitory effect on the proliferation of lung cancer cell lines increased. The median inhibition of small cell lung cancer was 95nM, UCN-01 was 588nM, and non-small cell lung cancer was 188nM and 1029nM, respectively. ST and UCN -01 can inhibit the phosphorylation of Rb protein in lung cancer cells. Conclusion Protein phosphorylase inhibitors inhibit lung cancer cell proliferation by inhibiting phosphorylation of Rb protein