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为查明非典型肺炎的病原体 ,作者以死亡患者肺组织感染KM乳鼠和VeroE6细胞 ,对发病鼠的主要脏器作病理学检查 ,对出现病变的培养细胞作超微结构观察 ,对细胞培养上清液作电镜负染检查。结果发现 ,患者肺组织可致乳鼠发病死亡 ,病变主要表现在肺脏和肝脏 ,在肺组织中检出病毒。在感染的培养细胞中发现大量病毒 ,在细胞培养液中也检出病毒 ,检出的病毒大多呈球形 ,直径 90～12 0nm ,其形态和细胞内分布与冠状病毒相一致 ,表明冠状病毒为非典型性肺炎的病原体
In order to find out the pathogen of SARS, the author inoculated KM suckling mice and Vero E6 cells in the lung tissue of the deceased patients, and made pathological examination on the main organs of the diseased mice. The ultrastructural changes of the cultured cells were observed, Supernatant electron microscopy negative staining. The results showed that the patient’s lung tissue can cause the death of suckling mice, lesions mainly in the lungs and liver, the virus detected in the lung tissue. Infected cells found in a large number of viruses, viruses were detected in the cell culture medium, most of the detected viruses were spherical, diameter 90 ~ 120nm, its morphology and intracellular distribution consistent with the coronavirus, coronavirus Atypical pneumonia pathogens