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目的 研究慢性癫痛大鼠点燃时海马星形胶细胞的激活情况。方法 采用马桑内酯慢性癫痫大鼠模型 ,观察大鼠点燃后海马 NF-κBp6 5和胶质原纤维酸性蛋白 (glial fibrillary acidic protein,GFAP)免疫细胞化学反应(im munoreactivity,IR)的变化。结果 点燃后 1h,海马 CA1区 GFAP- IR开始增强 ,4～ 8h可观察到 GFAP- IR阳性细胞数量增多并明显浓染 ,这种强 GFAP- IR持续至点燃后 2 4h;点燃后 1h,NF- κBp6 5即可在海马 CA1区神经元和胶质细胞内表达 ,主要位于胞核内 ,至 8h阳性神经元细胞核基本消失而可见大量 NF- κBp6 5 - IR阳性的胶质细胞 ;双重免疫细胞化学方法显示 GFAP/ NF- κBp6 5 - IR阳性细胞在点燃后 1h即可观察到 ,4h达最高峰 ,2 4h恢复至对照水平。结论 马桑内酯慢性致痫大鼠点燃时星形胶质细胞表现出一种早期而持续的激活 ,提示反复激活的星形胶质细胞对癫痫的复发可能起重要的作用。
Objective To investigate the activation of astrocytes in hippocampus of rats with chronic epilepsy. Methods Chronic epilepsy rats model was used to observe the changes of the immunommunochemical reaction (im munoreactivity, IR) of NF-κBp65 and glial fibrillary acidic protein (GFAP) in the hippocampus of rats. Results After 1 h of ignition, the GFAP-IR of hippocampal CA1 region began to increase, and the number of GFAP-IR positive cells increased significantly after 4 ~ 8 h. The strong GFAP-IR lasted for 24 h after ignition. 1 h after ignition, NF - κBp65 could be expressed in neurons and glial cells in CA1 area of hippocampus, mainly located in the nucleus. The nuclei of neurons disappeared in 8 hours and a large number of NF - κBp65 - IR positive glial cells were seen. Double immune cells The chemical method showed that GFAP / NF-κBp6 5 - IR positive cells were observed at 1h after ignition, reaching the peak at 4h and returned to the control level at 24 h. Conclusion The astrocytes of chronic epileptic rats with epilepsy induced by early-onset coronatine showed an early and sustained activation, suggesting that repeated activation of astrocytes may play an important role in the relapse of epilepsy.