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研究了慢性应激对小鼠脑中神经营养因子蛋白表达的影响 ,并探讨了性激素降低是否参与此过程 .性成熟完整雄性小鼠和去睾丸小鼠接受慢性应激 6 0d ,完整动物海马和大脑皮层中神经营养因子3(NT 3)和脑源性神经营养因子 (BDNF)蛋白水平显著降低 ,而且NT 3在海马齿状回的降低呈现某种程度的特异性 ,此外 ,齿状回颗粒细胞的形态退化在整个脑中也最为明显 ;去睾丸应激动物海马和大脑皮层中BDNF和NT 3蛋白水平较完整动物进一步降低 ,尤其是海马齿状回NT 3蛋白水平的降低与其他脑区相比表现出明显的特异性 ,同样 ,慢性应激引起的大脑皮层和海马特别是齿状回的神经元退化进一步被去睾丸恶化 .结果表明 ,慢性应激可抑制脑中NT 3和BDNF蛋白表达 ,去睾丸可使这种变化进一步加剧 ,海马齿状回对应激神经毒性较为敏感 ,提示 :脑中BDNF和NT 3蛋白水平降低与慢性应激过程中大脑形态退化密切相关 ;性激素降低在应激诱导的大脑退化过程中可能扮演着重要角色
The effects of chronic stress on the expression of neurotrophic factor protein in the brain of mice were studied and whether the decrease of sex hormones was involved in this process was studied.The sexual maturation of intact and testicular mice received chronic stress for 60 days, Neuronal trophic factor 3 (NT 3) and brain derived neurotrophic factor (BDNF) protein levels were significantly decreased in the cerebral cortex, and NT 3 degeneration in the hippocampal dentate gyrus showed some degree of specificity. In addition, the dentate gyrus granule Degradation of cells was most obvious in the whole brain. BDNF and NT 3 protein levels in hippocampus and cortex of testicular stress-induced animals were further lower than those in intact animals, especially in hippocampal dentate gyrus. Compared with other brain regions Similarly, chronic neuron degeneration in the cortex and hippocampus, especially the dentate gyrus, was further exacerbated by orchids in response to chronic stress.The results show that chronic stress inhibits NT 3 and BDNF proteins in the brain Expression or detoxication further aggravated this change. Hippocampal dentate gyrus was more sensitive to the stress neurotoxicity, suggesting that the decrease of BDNF and NT 3 protein in the brain was associated with chronic stress The degradation process is closely related to brain morphology; sex hormones in the brain to reduce stress-induced degradation processes may play an important role