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目的:探讨4’-去甲峨参内酯(0号衍生物)作用于Hela、MG-63、Hep G2、A549四种肿瘤细胞后,对Bcl-2、Bax基因表达、细胞周期及凋亡产生的影响。方法以0号衍生物作为观察组,0.1%DMSO作为空白对照组,紫杉醇作为阳性对照组,分别作用48 h后采用ELISA试剂盒检测对四种肿瘤细胞Bcl-2、Bax表达的影响;采用流式细胞仪PI单标法检测细胞周期进程及凋亡的影响。结果 ELISA试剂盒检测表明,观察组及阳性对照组与空白对照组比较Bcl-2/Bax比值均明显降低,具有显著性差异(P<0.05);流式细胞仪检测0号衍生物作用四种肿瘤细胞48h后对细胞周期进程及凋亡的影响,结果表明G0/G1期细胞百分数降低,G2/M期细胞百分数基本不变,而S期细胞百分数上升,说明0号衍生物阻滞肿瘤细胞于S期,且sub-G1细胞亚二倍体凋亡峰增加,进一步说明其诱导凋亡的作用。结论 0号衍生物通过上调Bax、下调Bcl-2,促进肿瘤细胞凋亡;阻滞肿瘤细胞于S期,sub-G1峰增加,显示其具有诱导细胞凋亡的作用。
OBJECTIVE: To investigate the effects of 4’-normethinolide (0 derivative) on the expression of Bcl-2 and Bax, cell cycle and apoptosis in Hela, MG-63, Hep G2 and A549 tumor cells Impact. Methods 0 # derivatives as the observation group, 0.1% DMSO as the blank control group, paclitaxel as the positive control group, respectively 48 h after the use of ELISA kit to detect the four tumor cells Bcl-2, Bax expression; using flow Cytometry PI Monoclonal Assay to Detect Cell Cycle and Apoptosis. Results The results of ELISA kit showed that the ratio of Bcl-2 / Bax in the observation group and the positive control group was significantly lower than that in the blank control group (P <0.05), and the effect of the 0 derivative was detected by flow cytometry The results showed that the percentage of cells in G0 / G1 phase decreased, the percentage of cells in G2 / M phase remained almost unchanged, while the percentage of cells in S phase increased, indicating that derivative 0 blocked tumor cells In S phase, the sub-G1 sub-diploid apoptotic peak increased, further illustrating its role in inducing apoptosis. Conclusion 0 derivative can promote the apoptosis of tumor cells by up-regulating Bax and down-regulating Bcl-2. It can block tumor cells in S phase and increase the sub-G1 peak, which indicates that it has the effect of inducing apoptosis.