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中国是世界产棉大国,由于虫害的连年发生,给棉花生产造成了巨大的经济损失。为解决制约棉花生产发展的这一重大难题,笔者承担了国家“863”棉花抗虫基因工程研究课题。目前取得的新进展如下。 1.转Bt杀虫基因抗虫棉研究根据Bt杀虫蛋白质的氨基酸序列,按照植物优化密码子,采用基因工程技术,人工合成了GFMCryIA杀虫基因,并将杀虫基因重组到带有多个植物高效表达元件的pGΩ4A载体上,构
China is a big cotton-producing country in the world. As a result of successive pests, it has caused huge economic losses to cotton production. In order to solve this major problem restricting the development of cotton production, the author undertook the national “863” cotton insect-resistant genetic engineering research project. The new progress made so far is as follows. 1. Transgenic Bt insect-resistant cotton study Bt insecticidal protein based on the amino acid sequence, in accordance with plant-optimized codons, the use of genetic engineering technology, artificial synthesis of GFMCryIA insecticidal genes, and the insecticidal gene recombinant with multiple Plant pGΩ4A efficient expression element on the vector