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狗枣猕猴桃(Actinidiakolomikta)在远缘杂交过程中容易落果,无法获得杂交后代,针对这一问题以中科院武汉植物园猕猴桃资源圃中狗枣猕猴桃为母本,4倍体中华猕猴桃雄株(A.chinensis)为父本进行种间远缘杂交,授粉后29、45d小心剔取胚珠接种于培养基上,4℃条件下暗处理2周后转移至光下培养,每4周继代1次,当幼苗生长高度达3cm以上且根系发达时炼苗移栽。结果显示,培养基MS+GA30.2mg/L+NAA0.2mg/L+ZT1.0mg/L上,胚珠的萌发率最高,达到36.67%,在培养基MS+GA30.5mg/L+NAA0.5mg/L+6-BA2.0mg/L上,幼苗长势好,而培养基1/2MS+IBA0.7mg/L对根系的发育具有良好的促进作用。
Actinidia kolomikta was easy to deciduous during distant hybridization and could not obtain hybrid progeny. To solve this problem, A. chinensis was used as the female parent in the kiwifruit resource garden of Wuhan Botanical Garden of Chinese Academy of Sciences. ) Were crossed with their parents. After pollination, the ovules were picked carefully on the medium at 29 and 45 days after pollination. After 2 weeks of dark treatment at 4 ℃, they were transferred to light and subcultured once every 4 weeks. When the seedlings grew Height of more than 3cm and developed rooted seedlings transplanting. The results showed that the highest germination rate of ovules was 36.67% on the medium of MS + GA30.2mg / L + NAA0.2mg / L + ZT1.0mg / L, /L+6-BA2.0mg/L, the seedlings grew well, while the medium 1 / 2MS + IBA0.7mg / L had a good promotion on the root development.