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目的探讨维生素E在噪声暴露引起的大白鼠听力损伤有明显的保护作用及机制。方法健康雄性纯种Wistar大鼠9只,鼠龄2～3个月,体质量180～200g,由长春市高新开发区实验动物中心提供(清洁级)。无噪声暴露及耳毒性药物使用史,耳廓反射正常,实验前清洁外耳道,显微镜下检查均无中耳炎。以听阈、耳蜗基底膜铺片及免疫荧光染色为指标,观察大白鼠在稳态噪声持续暴露之前30d至暴露后7d,灌维生素E及生理盐水,对大白鼠听觉脑干电位分析和耳蜗外毛细胞(OHC)丢失率分析及NF-κB在耳蜗毛细胞中表达定量分析。结果维生素E在噪声暴露后大白鼠听力阈值明显低于单纯噪声组,OHC丢失率也明显降低,免疫荧光染色结果显示VE干预组的耳蜗细胞表达NF-κB的强度低于单纯噪声组(P<0.05)。结论维生素E在噪声暴露引起的大白鼠听力损伤有明显的保护作用,通过上调耳蜗毛细胞中NF-κB的表达。
Objective To investigate the protective effect and mechanism of vitamin E on hearing loss induced by noise in rats. Methods Nine healthy male Wistar rats, aged 2 to 3 months with a body weight of 180 ~ 200g, were provided by Experimental Animal Center of Changchun High-tech Development Zone (clean grade). Noise-free exposure and history of ototoxic drug use, auricle reflexes normal, external auditory canal before the experiment, no otitis media under the microscope. The auditory threshold, cochlear basilar membrane membrane and immunofluorescence staining were used as indexes to observe the changes of auditory brainstem response and cochlear outer hair in rats after exposure to steady-state noise for 30 days to 7 days after exposure to vitamin E and saline. Analysis of cell loss rate (OHC) and expression of NF-κB in cochlear hair cells. Results The hearing threshold of vitamin E in rats exposed to noise was significantly lower than that of rats in simple noise group and the loss of OHC was also significantly decreased. Immunofluorescence staining showed that the intensity of NF-κB expression in cochlear cells in VE group was lower than that in simple noise group (P < 0.05). Conclusion Vitamin E has a significant protective effect on hearing loss induced by noise exposure in rats. It can increase the expression of NF-κB in cochlear hair cells.