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为了进一步探讨黄瓜嫁接苗比自根苗抗病抗逆性好、光合能力强的机理,在塑料大棚和高温消毒基质栽培条件下,用双向电泳(2-DE)技术和质谱(MS)技术研究了‘黑籽南瓜’与‘津优1号’黄瓜嫁接苗和‘津优1号’黄瓜自根苗叶片的蛋白质变化。嫁接后分别于苗期、开花期和结果期测定嫁接苗和自根苗叶片的蛋白质。结果表明,嫁接苗叶片中新产生了两类蛋白质:能提高抗病抗逆能力的R蛋白(RGC693蛋白)和促进萜烯类物质合成的鲨烯合酶,能促进叶绿体合成的辅酶和提高光能利用率的捕光叶绿素a/b结合蛋白。它们的出现可以说明嫁接黄瓜的抗病抗逆能力和光合能力优于自根苗的原因。
In order to further explore the mechanism of cucumber grafted seedlings resistant to disease and stress than the rooted seedlings, photosynthetic capacity of the mechanism of plastic greenhouse and high temperature sterilization substrate culture conditions, using two-dimensional electrophoresis (2-DE) technology and mass spectrometry (MS) technology The Change of Proteins in Cucumber Seedling Grafted on ’Black Seed Pumpkin’ and ’Jinyou 1’ and ’Zi Jin No.1’ Cucumber Seedling. After grafting, the proteins of grafted seedlings and self-rooted seedlings were determined at seedling stage, anthesis stage and fruit stage respectively. The results showed that two kinds of new proteins were produced in the leaves of grafted seedlings: the R protein (RGC693 protein) which can improve disease resistance and resistance, the squalene synthase which can promote the synthesis of terpenoids, the coenzyme that can promote chloroplast synthesis, Utilization rate of light harvesting chlorophyll a / b binding protein. Their emergence can explain the grafted cucumber disease resistance and photosynthetic ability than the root cause of the seedling.