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以组织培养和土壤播种的未经层积处理的五味子种子为材料,检测胚乳和外源赤霉素(GA3)对发芽影响的结果表明,在不加生长调节物质的MS培养基上,去掉外种皮带全胚乳的种子发芽率只有2.7%,而带1/2和1/3胚乳的种子发芽率则分别为46%和70.6%;培养基中添加GA3,可提高去掉外种皮带全胚乳和带1/2胚乳种子的发芽率,而对带1/3胚乳的种子则无效果。土壤播种时,用10~1000mg·L-1GA3对去掉外种皮种子进行1~10d前处理,其中100mg·L-1GA3前处理10d的发芽率最高,达到38.7%。
The effects of endosperm and exogenous gibberellic acid (GA3) on the germination of the Schisandra chinensis seeds which were not treated by the layering were observed in tissue culture and soil sowing. The results showed that on the MS medium without the growth regulator, Seed germination rate of whole endosperm germ was only 2.7%, while those with 1/2 and 1/3 endosperm germination rate were 46% and 70.6%, respectively. Adding GA3 to the medium could improve the removal of whole endosperm and Germination rates with 1/2 endosperm seeds are not effective with 1/3 endosperm seeds. Soil sowing, with 10 ~ 1000mg · L-1GA3 to remove the outer seed coat seeds for 1 ~ 10d pretreatment, 100mg · L-1GA3 pretreatment 10d the highest germination rate, reaching 38.7%.