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目的:探索血清骨桥蛋白(OPN)作为肝细胞癌(HCC)生物标志的应用价值。方法:应用ELISA法检测70例HCC患者、65例慢性肝脏疾病(CLD)患者及65名健康人血清OPN、甲胎蛋白(Alpha-fetoprotein,AFP)浓度。结果:HCC组血清OPN浓度(中位数975ng/mL,范围131～5 920ng/mL)明显高于CLD组(352ng/mL,18～1 875ng/mL,P<0.001)或健康人组(103ng/mL,8～984ng/mL,P<0.001)。在HCC组,血清OPN浓度随着Child-Pugh分级(F=8.053,P=0.001)及肿瘤分期(P=0.01)的升高而显著升高,且血清OPN浓度与肿瘤包膜的完整性明显相关(P=0.001)。OPN诊断HCC的敏感度、特异度及临界值分别为87.1%、75.4%和642.5ng/mL。OPN曲线下面积(0.895±0.028)比AFP(0.817±0.04)大(P<0.01),这提示OPN有优越的诊断效能。结论:血浆中OPN浓度可以作为诊断HCC潜在的参考分子指标之一。
Objective: To explore the value of serum osteopontin (OPN) as biomarker of hepatocellular carcinoma (HCC). Methods: Serum levels of OPN and Alpha-fetoprotein (AFP) in 70 patients with HCC, 65 patients with chronic liver disease (CLD) and 65 healthy people were detected by ELISA. Results: The serum level of OPN in HCC group was significantly higher than that in CLD group (median 975ng / mL, 131 ~ 5 920ng / mL) (352ng / mL, 18 ~ 1875ng / mL, P <0.001) / mL, 8 ~ 984 ng / mL, P <0.001). In HCC group, the serum OPN concentration was significantly increased with the increase of Child-Pugh classification (F = 8.053, P = 0.001) and tumor stage (P = 0.01), and the serum OPN concentration and tumor capsule integrity were significantly Related (P = 0.001). The sensitivity, specificity and critical value of OPN in diagnosing HCC were 87.1%, 75.4% and 642.5 ng / mL, respectively. The area under the OPN curve (0.895 ± 0.028) was larger than that of AFP (0.817 ± 0.04) (P <0.01), suggesting that OPN has superior diagnostic efficacy. Conclusion: The concentration of OPN in plasma can be used as one of the potential molecular markers for diagnosis of HCC.