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利用OTC(开顶箱)法模拟未来CO2浓度,于CO2倍增浓度(700μmol·mol-1)和正常空气CO2浓度(≈350μmol·mol-1)条件下,测定了沈阳市区油松(Pinustabulaeformis)针叶超氧自由基(O2.-)产生速率、过氧化氢(H2O2)含量、超氧化物歧化酶(SOD)与抗坏血酸-谷胱甘肽循环(ASA-GSHcycle)主要酶活性动态变化,探讨高浓度CO2对油松抗氧化酶活性的影响。结果表明:在短期(60d)内CO2浓度倍增使油松超氧自由基(O2.-)产生速率与过氧化氢(H2O2)含量减少,而SOD、抗坏血酸过氧化物酶(APX)、单脱氢抗坏血酸还原酶(MDAR)、脱氢抗坏血酸还原酶(DHAR)、谷胱甘肽还原酶(GR)活性升高;植株抗氧化能力增强,对活性氧清除能力提高;但长期(70d以上)CO2浓度倍增处理则可能使试验结果发生逆转。
The future CO2 concentration was simulated by the OTC (open top box) method. Pinus tabulaeformis population in Shenyang was measured under doubled CO2 concentration (700μmol · mol-1) and normal air CO2 concentration (≈350μmol · mol-1) Dynamic changes of major enzyme activities in the production rate of needle superoxide radical (O2.-), hydrogen peroxide (H2O2), superoxide dismutase (SOD) and ascorbate-glutathione cycle (ASA-GSHcycle) Effect of High Concentration CO2 on Antioxidant Enzyme Activities of Pinus tabulaeformis. The results showed that in the short term (60d), doubled CO2 concentration reduced the production rate of O2.- and hydrogen peroxide (H2O2), while SOD, ascorbate peroxidase (APX) The activities of MDAR, DHAR and GR increased. The antioxidant capacity of plants increased and the ability of scavenging reactive oxygen species increased. However, long-term (more than 70 days) CO2 Concentration doubling may reverse the test results.